Available Resources:


OnePot PURE System:

All OnePot PURE system plasmids are available from Addgene.

RF1 is currently not availalbe on Addgene but RF2 should be sufficient. If RF1 is required the plasmid can be requested directly from Prof. Shimizu.

Strains for ribosome purification: RB1 strain can be obtained from Professor Wang (Columbia University, USA) and A19 is availalbe from CGSC.

External repositories:

protocols.io (protocols)

GitHub (software)

Zenodo.org (data, design files, etc.)

Plasmid repositories:

Link to Sebastian Maerkl Lab Plasmids Find Maerkl Lab Plasmids

If you can't find what you are looking for on addgene, please contact us directly.